![]() ![]() Homogeneous population (monoclonal T cells recovered 4 d after antigen stimulation), using either this method or conventional We evaluated the impact of this approach on functional genetic read-outs by studying an apparently The precise quantification of the absolute number of mRNA molecules per cell, ranging from 2 to 1.28×10 9 for each individual gene. Abundance relationships are maintained, allowing Uniform and maximized amplification conditions for all genes are provided. This method has broad application, in different species and any type of gene combination. We here describe a new reverse transcription-polymeraseĬhain reaction (RT-PCR) approach allowing the simultaneous quantification of the expression of 20 genes in the same singleĬell. ![]() Of all types of biological events and/or differentiation processes. ![]() Individual cell behavior requires multiple gene expression analyses of single cells, and may be fundamental for the understanding Population, overlooking possible cell-to-cell heterogeneity that could lead to different cell behaviors/cell fates. Therefore, they determine the average gene expression within a So far, theseĪssessments can only be performed at the population level. Quantitative gene expression analysis aims to define the gene expression patterns determining cell behavior. ![]()
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